ISO INTERNATIONAL STANDARD 21571 First edition 2005-02-15 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction Produits alimentaires - Methodes d'analyse pour la detection des organismes génetiquement modifiés et des produits dérives - Extraction des acides nucléiques Reference number ISO 21571:2005(E) Copyright International Organization for Standardization @ ISO 2005 itted without license from IHS Not for Resale ISO 21571:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below ?ISO2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland yemainagazainandardizato @ISO2005-All rightsreserved mittedwithoutlicense from IHS Not for Resale ISO 21571:2005(E) Contents Page Foreword IV Introduction 1 Scope 2 Normative references : Principle 3.1 General 3.2 DNA extraction 3.3 DNA quantitation 4 General laboratory requirements 5 Procedure..... 5.1 Preparationofthetestportion. 5.2 DNA extraction/purification... 5.3 QuantitationoftheextractedDNA 5.4 Stability of extracted DNA.... 6 Interpretation 7 Test report. Annex A (informative) Methods for DNA extraction... Annex B (informative) Methods for the quantitation of the extracted DNA. Bibliography Copyright International Organizaion for stadardizalon'ghts reserved ili nse from IHS Not for Resale ISO 21571:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies technicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeen InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2. adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. ISO 21571 was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analysis — Horizontal methods, in collaboration with Technical Committee ISO/TC 34 Agreement) Copyright Intern ional Organization for Standardization @ISO 2005-All rights reserved ISO 21571:2005(E) Introduction The search for genetically modified origin of ingredients is performed by means of the following successive (or simultaneous)steps.After sample collection,nucleic acids areextractedfrom thetest portion.Extracted quantified (if necessary), diluted (if necessary) and subjected to analytical procedures (such as PCR). These steps are detailed in this and the following International Standards: Iso 21568, Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products- Sampling. Iso 21569, Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods. IsO 21570, Foodstuffs - Methods of