ISO INTERNATIONAL STANDARD 21569 First edition 2005-06-15 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods Produits alimentaires - Methodes d'analyse pour la detection des organismes genétiquement modifiés et des produits derivés Methodes qualitatives basées sur I'utilisation des acides nucleiques Reference number ISO 21569:2005(E) Copyright International Organization for Standardization @ISO2005 itted without license from IHS Not for Resale ISO21569:2005(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below @ISO2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland Copyightinteratinal organization for Standardization @ ISO 2005 - All rights reserved nitted without licensefromIHS Not for Resale ISO 21569:2005(E) Contents Page Foreword. Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle of the method. 5 Reagents 6 Apparatus and equipment 7 Procedure. 8 Interpretation.... 6 9 Expression of results and quality assurance 10 Test report .... Annex A (informative) Target-taxon-specific methods.. Annex B (informative) Screening methods.. Annex C (informative) Construct-specific methods . .40 Annex D (informative) Event-specific methods.... Bibliography... Copyright International Organizaion for standardizalor ghts reserved ii from IHS Not for Resale ISO 21569:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2. adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote ISO 21569 was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analysis - Horizontal methods, in collaboration with Technical Committee ISO/TC 34 Agreement) ional OrganizationforStandardization @ISO2005-All rightsreserved ISO 21569:2005(E) Introduction (orsimultaneous)steps.Aftersamplecollection,nucleicacidsareextractedfromthetestportion.Extracted nucleicacidscanbefurtherpurified,simultaneouslyoraftertheextractionprocess.Afterwards,theyare quantified (if necessary), diluted (if necessary) and subjected to analytical procedures (such as PCR). These steps are detailed in this International Standard and in the following series of International Standards with the general title Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products: Sampling (ISO 21568); Quantitative nucleicacid based methods (isO21570) Nucleic acid extraction (IsO 21571) Further information about general requirements and definitions involving the steps cited above are collected in ISO24276.