ISO INTERNATIONAL STANDARD 21427-1 First edition 2006-08-01 Water quality Evaluation of genotoxicity by measurement of the induction of micronuclei- Part 1: Evaluation of genotoxicity using amphibian larvae Qualite de I'eau - Evaluation de la genotoxicité par le mesurage de I'inductiondemicronoyaux Partie 1: Evaluation de la génotoxicité a I'aide de larves d'amphibiens Reference number ISO 21427-1:2006(E) @ ISO 2006 ISO 21427-1:2006(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The Iso Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation ?ISO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2006 - All rights reserved ISO 21427-1:2006(E) Contents Page Foreword. Introductior 1 Scope 2 Normative references 3 Termsanddefinitions. 4 Principle 5 Testenvironment 6 Reagents 7 Apparatus 8 Treatmentandpreparationofsamples. 8.1 Waters, effluents, leachates and eluates to be tested .. 8.2 Substances to be tested . 9 Procedure.. 9.1 Selection of concentrations.. 9.2 Conducting the test. 9.3 Reading of the histological preparations. 10 Expression of results 10.1 Presentation of results. 10.2 Processing of results 10.3 Interpretation of results. 11 Validity of the test 12 Test report . Annex A (normative) Data specific to Pleurodeles waltl. Annex B (normative) Breeding ...... 10 Annex C (informative) Example of a statistical method for processing results Bibliography... ion'ghts reserved ili ISO 21427-1:2006(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through sO technical committees. Each member body interested in a subject for which a technical committee has been non-governmental, in liaison with iso, also take part in the work. Iso collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 21427-1 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Iso 21427 consists of the following parts, under the general title Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei: Part 1: Evaluation of genotoxicity using amphibian larvae Part 2: Mixed population method using the cell line V79 @ IS0 2006 - All rights reserved ISO 21427-1:2006(E) Introduction Environmental protection calls for taking into account the genotoxic hazards likely to concern the different populat