BS EN ISO BRITISH STANDARD 20645:2004 Textile fabrics Determination of antibacterial activity- Agar diffusion plate test The European Standard EN IS0 20645:2004 has the status of a British Standard ICS 59.080.30 BSi British Standards BS EN ISO 20645:2004 National foreword This British Standard is the official English language version of EN ISO 20645:2004.It is identical with ISO 20645:2004. The UK participation in its preparation was entrusted to Technical Committee TCI/80, Textiles Chemical testing, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index", or by using the “Search" facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not ofitself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, pages 2 to 13 and a back cover. The BSI copyright notice displayed in this document indicates when the documentwaslastissued. Amendments issued since publication This British Standard was published under the authority Amd. No. Date Comments of the Standards Policy and Strategy Committee on 20December2004 C BSI 20 December 2004 ISBN 0 580 45126 7 EN ISO 20645 EUROPEANSTANDARD NORME EUROPEENNE EUROPAISCHE NORM December 2004 ICS59.080.30 English version Textile fabrics - Determination of antibacterial activity - Agar diffusion plate test (1SO 20645:2004) Etoffes - Controle de I'activite antibacterienne - Essai de Textile Flachengebilde - Prufung der antibakteriellen diffusion sur plaques de gelose (IsO 20645:2004) Wirkung - Agarplattendiffusionstest (ISO 20645:2004) ThisEuropeanStandard wasapprovedbyCENon2September2004. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standardsmaybeobtained onapplicationtotheCentral SecretariatortoanyCENmember. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany,Greece,Hungary,Iceland,Ireland,Italy,Latvia,Lithuania,Luxembourg,Malta,Netherlands,Norway,Poland,Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. cer EUROPEANCOMMITTEE FORSTANDARDIZATION COMITEEUROPEENDENORMALISATION EUROPAISCHESKOMITEEFURNORMUNG Management Centre:ruedeStassart,36B-1050Brussels @2004CEN All rights of exploitation in any form and by any means reserved Ref.No.ENISO20645:2004:E worldwidefor CEN national Members. ENISO20645:2004 (E) Contents Page Foreword Introduction .. 1 Scope .5 2 Terms and definitions. 3 Safety precautions.. .5 4 Principle. 5 Apparatus, reagents and culture media 5.1 Apparatus.. 5.2 Reagents and culture media... 6 Test bacteria. 7 Preparation of the bacteria cultures 7.1 General.. 7.2 Culturing with lyophilized bacteria 7.3 Culturing from the a