ISO INTERNATIONAL STANDARD 19458 First edition 2006-08-01 Water quality Sampling for microbiological analysis Qualite de I'eau - Echantillonnage pour analyse microbiologique Reference number ISO 19458:2006(E) @ ISO 2006 ISO19458:2006(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The Iso Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation ?ISO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ISO 2006-All rights reserved ISO19458:2006(E) Contents Page Foreword. iv Introductior 1 Scope 2 Normative references 3 Sampling point. 4 Sampling technique. 5 Transport and storage. Annex A (informative) A priori determination of the number of samples to analyse to determine the mean concentration of microbes in water with a given confidence, for quantitative determination derived by cultivation of microorganisms ... 13 Annex B (informative) Recommended (R) and acceptable (A) values for maximum sample storage times including transport time and temperatures unless otherwise specified in specific Bibliography 'ghtsreserved ili ISO 19458:2006(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through sO established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with isO, also take part in the work. isO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting.Publicationasan International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights: IsO shall not be held responsible for identifying any or all such patent rights. ISO19458 was prepared by Technical Committee ISO/TC 147,Water quality, Subcommittee SC 4, Microbiological methods. @ ISo 2006 - All rights reserved ISO 19458:2006(E) Introduction Sampling features depend on the objective of sampling, but also on the nature of the sample. Microorganisms are living organisms. In addition, when they are introduced into water, they do not form a perfect solution, but a suspension with an inherent degree of variability. Sampling objectives may serve different purposes, which are described in the IsO 5667 series of standards (ISO 5667-1, ISO 5667-2 and ISO 5667-3): a) determination of the compliance of a water with a regulatory quality specification; b) characterization of any contamination, its level (mean) and its variations: 1) what is its random variation? 2) is there a trend? 3) are there cycles? c) identi