ISO INTERNATIONAL STANDARD 18145 First edition 2003-07-15 Environmental tobacco smoke Determination of vapour phase nicotine and 3-ethenylpyridine in air Gas- chromatographic method Fumée de tabac ambiante - Dosage de la nicotine et de la 3-éthenylpyridine en phase vapeur dans I'air- Methode par chromatographie en phase gazeuse Reference number ISO 18145:2003(E) ISO @ ISO 2003 d by IHS under lic ted without license from IHS Not for Resale ISO18145:2003(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO2003 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland Copyriahtinteratinal organization for Standardization @IsO2003-All rightsreserved py IHS unde ermitted without license from IHS Not for Resale ISO 18145:2003(E) Contents Page Foreword. iv Introduction 1 Scope. 2 Normative references ... 3 Terms and definitions. 4 Principle 5 Limits and detection . 6 Reagents 7 Apparatus. 8 Sampling procedure .. 8.1 Calibration of the personal sampling pump.... 8.2 Sorbent tube and personal sampling pump preparation. 8.3 Sample collection... 9 Analytical procedure. 9.1 General.... 9.2 Sorbent resin desorption and extraction... 9.3 Loading autosampler.... 9.4 Gas chromatographic (GC) determination of nicotine and 3-ethenylpyridine 10 Expression of results... 9 10.1 Caiculation of desorption efficiency .... 10.2 Calculation of analyte concentration in the sample... 10.3 Calculation of analyte content in the air.. 10 11 Laboratory performance criteria and quality assurance 10 12 Repeatability and reproducibility.. .10 13 Test report.. 11 Annex A (informative) Laboratory performance criteria: Quality assurance measures 12 Bibliography .. 14 Copyrght International Organizaion for Standardization rights reserved ili Not for Resale ISO18145:2003(E) Foreword (isO member bodies). The work of preparing International Standards is normally carried out through IsO technicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeen InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 18145 was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products ional Organizationfor Standardization @ISO2003-Allrightsreserved ISO 18145:2003(E) Introduction Nicotine and 3-ethenylpyridine (3-EP) are commonly used tracers for environmental tobacco smoke (ETS). Nicotine and 3-EP are highly selective for tobacco smoke and both have been used as markers of ETs in indoor air. Among the attributes of