ISO INTERNATIONAL STANDARD 17375 First edition 2006-06-01 Animal feeding stuffs Determination of aflatoxin B1 Aliments des animaux -Dosage de I'aflatoxine By Reference number ISO 17375:2006(E) @ ISO 2006 ISO17375:2006(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The Iso Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation ? ISO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ISO2006-All rightsreserved ISO17375:2006(E) Contents Page Foreword. iv 1 Scope 2 Normative references 3 Principle 4 Reagents 5 Apparatus 6 Procedures 6.1 Conditioning of IACs 6.2 Extraction.... 6.3 Immunoaffinitycleanup 6.4 Post-columnderivatization 6.5 Calibration curve.. 6.6 Calculation.... 6.7 Spiking procedures for recovery determination ..... 7 Precision.. 7.1 Interlaboratorytest 7.2 Repeatability... 8 7.3 Reproducibility. 8 Test report 9 Annex A (informative) Results of an interlaboratory test. U Bibliography nizationfor Stand ion'ghts reserved ili ISO 17375:2006(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through IsO non-governmental, in liaison with iso, also take part in the work. Iso collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 17375 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs. @IsO2006-Allrightsreserved INTERNATIONAL STANDARD ISO 17375:2006(E) Animal feeding stuffs Determination of aflatoxin B, 1 Scope This International Standard specifies a method for the determination of aflatoxin B, in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization It is applicable to animal feeding stuffs with a fat content of up to 50 %. a signal-to-noise ratio of 6. NOTE The method is based on that given in Reference [1]. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated document (including any amendments) applies. IsO 3696:1987, Water for analytical laboratory use Specification and test methods 3 Principle A test portion is extracted with a solvent solution (acetone/water). The sample extract is filtered, diluted with water orphosphate-bufferedsaline toa specified solvent con