ISO INTERNATIONAL STANDARD 14673-1 IDF 189-1 Second edition 2004-02-01 Milk and milk products Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry Lait et produits laitiers Détermination des teneurs en nitrates et en nitrites- Partie 1: Methode par reduction au cadmium et spectrometrie Reference numbers ISO 14673-1:2004(E) IDF 189-1:2004(E) Copyright International Organization for Standardization @ ISO and IDF 2004 ted without license from IHS Not for Resale ISO 14673-1:2004(E) IDF189-1:2004(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. Neither the ISo Central Secretariat nor the IDF accepts any liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation and IDF national committees. In the unlikely event that a problem relating to it is found, please inform the ISO Central Secretariat at the address given below. @ ISO and IDF 2004 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO or IDF at the respective address below. ISO copyright office International Dairy Federation Case postale 56 : CH-1211 Geneva 20 Diamant Building·Boulevard Auguste Reyers 80.B-1030 Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32.2 733 04 13 E-mail [email protected] E-mail [email protected] Web www.iso.org Web www.fil-idf.org Published in Switzerland yemainagazainandardizato @ ISO and IDF 2004 - All rights reserved itted without license from IHS Not for Resale ISO 14673-1:2004(E) IDF 189-1:2004(E) Contents Page Foreword .iv 1 Scope. 1 2 Normative references 3 Terms and definitions.. 2 4 Principle, 5 Reagents .. 6 Apparatus 7 Sampling ..... 8 Preparation of test sample... 8.1 Dried milk and dried whey.... 8.2 Caseins and caseinates.... 8.3 8.4 Wheycheese 9 Procedure... 9.1 Preparation of the copperized cadmium column .... 9.2 Checking the reducing capacity of the column ..... 9.3 Regeneration of the column.... 9.4 Preparation of test portion... 9.5 Extraction and deproteination. 9.6 Reduction of nitrate to nitrite.... 8 9.7 Blank test..... 9 9.8 Determination 9.9 Preparation of calibration graph 9 10 Calculation and expression of results, 9 10.1 Nitrite content.... 10.2 Nitrate content. .10 11 Precision .10 11.1 General 10 11.2 Repeatability. 10 12 Test report.. 11 Bibliography 13 CopyrightIntematinalOganzanfrsandadiza4All rightsreserved ii ted withoutlicensefromIHS Not for Resale ISO 14673-1:2004(E) IDF 189-1:2004(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards ISOtechnicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehas been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/lECDirectives,Part2 adoptedbythetechnical committeesarecirculatedtothememberbodiesforvoting.Publicationas an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subjec