ISO INTERNATIONAL STANDARD 14592-1 First edition 2002-11-15 Corrected version 2003-08-01 Water quality - Evaluation of the aerobic biodegradability of organic compounds at low concentrations - Part 1: Shake-flask batch test with surface water or surface waterlsediment suspensions Qualité de I'eau -- Evaluation de la biodégradabilité aérobie des composés organiquesprésents enfaiblesconcentrations- Partie 1: Essai en lots de flacons agités avec des eaux de surface ou dessuspensionseauxdesurface/sédiments Reference number ISO 14592-1:2002(E) [so @ ISO 2002 Copyright International Organization for Standardization Not for Resa ISO 14592-1:2002(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF fle can be found in the General info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event @ISO2002 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic inthecountry of therequester. Iso copyright office Case postale 56:CH-1211Geneva 20 Tel.+ 4122 749 0111 Fax + 4122749 09 47 E-mail [email protected] Web www.iso.org PublishedinSwitzerland ii @ IS0 2002 - All rights reserved Not for Resale ISO 14592-1:2002(E) Contents Page Foreword. iv introduction. 1 Scope. 2 Normativereference 3 Terms,definitionsandsymbols 4 Principle.. 5 Reagents and media .. 6 Apparatus.. 7 Test environment and conditions.. 8 Procedure 6 Calculation... 10 Validity of the test 11 Test report. 13 Annex A (informative) Guidance on the use of 14c-labelled compounds ... 14 Bibliography. @ISO 2002-All rights reserved. ii Copyright International Organization for Standardization Not for Resale ISO 14592-1:2002(E) Foreword member bodies). The work of preparing International Standards is normally carried out through IsO technical Commission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the iSO/lE Directives, Part 3 The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standardrequiresapprovalbyatleast75%ofthememberbodiescastingavote Attention is drawn to the possibility that some of the elements of this part of isO 14592 may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 14592-1 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods Iso 14592 consists of the following parts, under the general title Water quality-- Evaluation of the aerobic biodegradabilityoforganiccompoundsatlowconcentrations: -Part1:Shake-flaskbatchtest with surfacewater or surfacewater/sediment suspensions Part 2: Continuous flow river model with attached biomass This corrected version of IsO 14592-1:2002 incorporates corrections to the reference given in the third item of the list in 8.2.1; the reference given in the penultimate line of 8.2.1; the reference given in the last line of the second paragraph of 8.4.1. iv @ Is0 2002 - All rights reserved Copyright International Organization for Standardizatior Not for Resale ISO14592-1:2002(E) Introduction This International Standard consists of two parts. Part 1 describes a die-away batch test for either surface water with or without added sediment in suspension simulating either a pelagicaquaticenvironmentora water-to-sedim