ISO INTERNATIONAL STANDARD 14183 First edition 2005-11-01 Animal feeding stuffs Determination of monensin, narasin and salinomycin contents Liquid chromatographic method using post-column derivatization Aliments des animaux - Détermination des teneurs en monensine, narasine et salinomycine Methode par chromatographie liquide utilisant la derivatisation post-colonne Reference number ISO 14183:2005(E) @ISO 2005 Not for Res ISO14183:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ?ISO2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's memberbody in the country of the requester. ISO copyright office Case postale 56. CH-1211Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland CopyightInternatinal organizationfor Standardization @ ISo 2005 - All rights reserved vithout license from IHS Not for Resale ISO 14183:2005(E) Contents Page Foreword 1 Scope 2 Normative references 3 Principle.. 4 Reagents. 5 Apparatus 6 Sampling. 7 Preparation of test sample.. 8 Procedure.... 8.1 Preparation of quality control sample 8.2 Extraction.. 8.3 HPLC analysis... 8.4 Determination... 9 HPLC confirmation . 9.1 General.. 9.2 Post-column derivatization with DMAB. 9.3 Hexane extraction ...... 10 10 Calculation of results 10 10.1 General. 10 10.2 Monensin. 10.3 Salinomycin, 10.4 Narasin... 12 10.5 Interpretation of confirmation data 13 11 Precision... 11.1 Interlaboratory test 11.2 Repeatability......... 11.3 Reproducibility. 14 11.4 Limit of quantitation ... 15 12 Test report 15 Annex A (informative) Results of interlaboratory test.. 16 Bibliography Copyright International Organizaion for standardizalon ghts reserved ii from IHS Not for Resale ISO 14183:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies technical committees. Each member body interested in a subject for which a technical committee has been InternationalElectrotechnicalCommission(iEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 14183 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feedingstuffs onalOrganizationfor Standardization ISO 2005 - All rights reserved Copyright Inter INTERNATIONAL STANDARD ISO 14183:2005(E) Animal feeding stuffs Determination of monensin, narasin and salinomycin contents Liquid chromatographic method using post-column derivatization 1 Scope d