ISO INTERNATIONAL STANDARD 13903 First edition 2005-05-15 Animal feeding stuffs Determination of amino acids content Aliments des animaux Determination de la teneur en acides amines Reference number ISO 13903:2005(E) ISO @ ISO 2005 ISO 13903:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. AdobeisatrademarkofAdobeSystems Incorporated Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In inn on 5 ISO2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 4122 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland i @ ISO 2005 - All rights reserved ISO 13903:2005(E) Contents Page Foreword iv 1 Scope 2 Principle. 2.1 Free amino acids 2.2 Total amino acids .. 3 Reagents and materials 4 Apparatus 5 Procedure... 5.1 Preparationoftestsample 5.2 Determination of free amino acids in feeding stuffs and premixtures 5.3 Determination of total amino acids . 5.4 Chromatography .... 6 Calculation of results. 7 Precision. 7.1 Interlaboratorytests 7.2 8 Repeatability. 7.3 Reproducibility. 8 Use of reference materials. 6 Observations on the method. Annex A (informative) Results of interlaboratory tests .. .10 AnnexB(informative)Examples of chromatograms. 15 Bibliography IsO2005-All rightsreserved ili ISO 13903:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2 The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 13903 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs ISO 13903 is based on Commission Directive 98/64/EC of September 1998 [1]. Ipllleu iv @ ISo 2005 - All rights reserved ISO 13903:2005(E) INTERNATIONAL STANDARD Animal feeding stuffs Determination of amino acids content 1 Scope This International Standard describes the determination of free (synthetic and natural) and total (peptide-bound and free) amino acids in feeding stuffs, using an amino acid analyser or HPLC equipment. It is applicable to the following amino acids: sum of cystine and cysteine; methionine; lysine; threonine; alanine; arginine; aspartic acid; glutamic acid; glycine; histidine; (nalllid isoleucine; leucine; phenylalanine; proline; serine; tyrosine; valine. The method does not distinguish between the salts of amino acids, nor does it differenti