BS ISO BRITISH STANDARD 13875:2005 Liquid milk Determination of acid-soluble T-lactoglobulin content - Reverse-phase HPLC method ICS 67.100.10 BSi NOCOPYINGWITHOUTBSIPERMISSIONEXCEPTASPERMITTEDBYCOPYRIGHTL British Standards BS ISO 13875:2005 National foreword This British Standard reproduces verbatim IS0 13875:2005 and implements it as the UK National Standard. The UK participation in its preparation was entrusted to Technical Committee AW/5, Milk and milk products, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled "International Standards Correspondence Index", or by using the “Search" facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not ofitself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the ISO title page, pages ii to v, a blank page, pages 1 to 14, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication This British Standard was Amd. No. Date published under the authority Comments of the Standards Policy and StrategyCommittee on 18November 2005 CBSI18November2005 ISBN 0 580 46215 3 BS IS0 13875:2005 INTERNATIONAL ISO STANDARD 13875 IDF 178 First edition 2005-02-01 Liquid milk Determination of acid- soluble E-lactoglobulin content Reverse-phase HPLC method Lait liquide Deétermination de la teneuren E-lactoglobulinesolubledansTacideMethodeparchromatographie liquidehauteperformanceenphaseinverse Reference numbers ISO 13875:2005(E) IDF 178:2005(E) iso = BS ISO 13875:2005 Contents Page Foreword 1 Scope 2 Normative references 3 Terms and definitions 4 Principle. 5 Reagents. 6 Apparatus 7 Sampling.. 8 Procedure... 8.1 Preparation of test portion 8.2 Preparationoftestsolution. 8.3 Preparationofreferenceportion... 8.4 Preparation of reference solutions for the multi-point calibration 8.5 Preparation of the reference solution for the “"single-point" calibration procedure. 8.6 HPLC determination... 8.7 Integration mode .. 9 Calculation and expression of results .... .8 9.1 Multi-point calibration. 9.2 Single-point calibration.. 9.3 Expressionofresults... 10 Standardization of the reference sample 10 10.1 General ..... 10.2 Preparation of the standard sample. 10 10.3 Determination of protein content.. 10.4 Determination of E-LG content in the reference sample 10 10.5 Calculation of the E-LG content. 10.6 Expression of results.. 11 Precision .. 11.1 Interlaboratory test 11.2 Repeatability 11.3 Reproducibility 12 Test report. 12 AnnexA(informative)Resultsofinterlaboratorytrials.. 13 Bibliography..... 14 ili BS IS0 13875:2005 Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards IsO technical committees. Each member body interested in a subject for which a technical committee has International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. adopted by the technical committees are circulated to the member bodies for voting. Publication as ar International Standard requires approval by at least 75 % of the member bodies casting a vote. rights. isO shall not be held responsible for identifying any or all such patent rights.