ISO INTERNATIONAL STANDARD 13829 First edition 2000-03-15 Water quality Determination of the genotoxicity of water and waste water using the umu-test Qualité de I'eau Détermination de la génotoxicité des eaux et des eaux réesiduairesalaidedel'essaiumu Reference number ISO 13829:2000(E) ISO @ ISO 2000 No r py IHS under lic itted without license from IHS Not for Resale ISO 13829:2000(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by isO member bodies. In the unlikely event ?ISO2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 734 10 79 E-mail [email protected] Web www.iso.ch Printed in Switzerland @ Iso 2000 - All rights reserved ermittedwithoutlicense from IHS Not for Resale ISO 13829:2000(E) Contents Page Foreword iv Introduction 1 Scope 2 Normative reference. 3 Terms and definitions , 4 Principle..... 5 Test organism and reagents. 6 Apparatus ... 7 Sample preparation and preservation . 8 Interferences ... 9 Test performance 10 Measurements... 11 Calculation and expression of results.. 12 Precision... 10 13 Validity criteria .. 14 Test report ... Annex A (informative) General definitions and terms regarding genotoxicity . Annex B (informative) Salmonella typhimurium TA1535/pSK1002. 12 Annex C (informative) Composition of the sample and control wells 13 Annex D (informative) Lowest value of the dilution series .. Annex E (informative) Checking the genotype.. Annex F (informative) Precision data from an interlaboratory study ... Annex G (informative) Final protocol for the umu-test Bibliography .. 8 ili by IHS under se from IHS Not for Resale ISO 13829:2000(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO committees. Each member body interested in a subject for which a technical committee has been established has liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights International Standard ISO 13829 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Annexes A to G of this International Standard are for information only ional Organization for Standardizatior ISO2000-All rightsreserved ISO 13829:2000(E) Introduction The genetically engineered bacterium Salmonella typhimurium TA1535/pSK1002 serves as a test organism. The bacteria are exposed under controlled conditions to different concentrations of the samples to be tested. The test is based on the capability of genotoxic agents to induce the umuC-gene in the Salmonella strain in response to genotoxic lesions in the DNA. different kinds of genotoxic substances. The induction of the umuC-gene is thus a measure for the genotoxic potential of the sample. Since the umuC-gene is fused with the lacZ-gene for β-galactosidase, th