ISO INTERNATIONAL STANDARD 1272 Second edition 2000-09-01 Essential oils Determination of content of phenols Huiles essentielles -Determination de la teneur en phenols Reference number ISO 1272:2000(E) SO @ ISO 2000 IHS under Not for Resale ISO 1272:2000(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this area. Adobe is atrademarkofAdobeSystems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event @ISO2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.ch Printed in Switzerland Iso2000-All rightsreserved itted without license from IHS Not for Resale ISO 1272:2000(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through iSO technical liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights International Standard ISO 1272 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (IsO 1272:1973), which has been technically revised. nafinai Organization for Standardizalionserved ii INTERNATIONAL STANDARD ISO 1272:2000(E) Essential oils Determination of content of phenols 1 Scope This International Standard specifies a method for the determination of the percentage, by volume, of phenols in essentialoils. NOTE 2Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of publications do not apply. However, parties to agreements based on this International Standard are encouraged to undated references, the latest edition of the normative document referred to applies. Members of isO and IEC maintain registers of currently valid International Standards. ISO 356:1996, Essential oils - Preparation of test sample. Iso 648, Laboratory glassware One-mark pipettes. IsO 1773, Laboratory glassware-Narrow-necked boiling flasks. 3Principle The phenolic compounds contained in a known volume of an essential oil are transformed into their alkaline phenol esters, which are soluble in the aqueous phase. The volume of the unabsorbed portion of the essential oil is measured. 4 Reagents Unless indicated otherwise, use only reagents of recognized analytical grade, and distilled or demineralized water or water of equivalent purity. 4.1 Tartaric acid, pulverized. 4.2 Potassium hydroxide, free from silica and alumina, 1