ISO INTERNATIONAL STANDARD 12193 Second edition 2004-02-01 Animal and vegetable fats and oils - Determination of lead by direct graphite furnace atomic absorption spectroscopy Corps gras d'origines animale et vegétale - Détermination de la teneur en plomb par spectrometrie d'absorption atomique directe avec four en graphite Reference number ISO 12193:2004(E) ISO @ ISO 2004 ISO 12193:2004(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is atrademarkof Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In @ISO2004 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ISO 2004-All rights reserved ISO 12193:2004(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies non-governmental, in liaison with isO, also take part in the work. isO collaborates closely with the InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 12193 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils. This second edition cancels and replaces the first edition (isO 12193:1994), which has been technically revised. @ ISO 2004 - All rights reserved ili INTERNATIONAL STANDARD ISO 12193:2004(E) Animal and vegetable fats and oils Determination of lead by direct graphite furnace atomic absorption spectroscopy Scope This International Standard specifies a method for the determination of trace amounts (> 0,o01 mg/kg) of lead in all types of crude or refined edible oils and fats. 2 Principle The oil or fat is atomized in a graphite furnace, with or without a platform, connected to an atomic absorption spectrometer which has been previously calibrated using standard solutions of an organo compound of lead. The metal content is measured from the observed absorbance at a wavelength of 283,3 nm. 3 Reagents Use only reagents of recognized analytical grade, unless otherwise specified, and distilled or demineralized water or water of equivalent purity. 3.1 Cyclohexane, analytical grade. 3.2 Lecithin, containing 2 % phosphorus. 3.3Matrix modifier: 2 % (by mass/volume) lecithin solution prepared by dissolving 2 g of lecithin (3.2) in 100 ml of cyclohexane (3.1). 3.4Blank oil, refined. greater than 0,001 mg/kg. 3.5Lead standard stock solution, c(Pb) = 10 mg/kg This is prepared by appropriate dilution of an organometallic standard with the blank oil (3.4) NOTE A suitable standard is available from the following companies:1) Continental Oil Company, Ponca City, Oklahoma, USA (Conostan, 5 0o0 mg/kg); Me