ISO INTERNATIONAL STANDARD 11348-2 Second edition 2007-12-01 Water quality Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 2: Method using liquid-dried bacteria Qualite de I'eau Determination de I'effet inhibiteur d'eéchantillons d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries luminescentes)- Partie 2: Methode utilisant des bactéries deshydratees Reference number ISO 11348-2:2007(E) @ ISO 2007 IHS under I without license from IHS Not for Resale ISO 11348-2:2007(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTEDDOCUMENT @ISO2007 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's:member body in the country of the requester. Iso copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2007 - All rights reserved ted without licensefrom IHS Not for Resale ISO 11348-2:2007(E) Contents Page Foreword. Introduction 1 Scope . 2 Normative references . 3 Principle... 4 Interferences 5 Reagents and materials . 6 Apparatus.. 7 Sampling and sample pretreatment... 8 Procedure 6 Evaluation. 10 Expression of results . 8 11 Criteria of validity.. .10 12 Precision 10 13 Test report. 10 Annex A (informative) Colour-correction method. 11 Annex B (informative) Dilution level D - Preparation of the dilution series.... .14 AnnexC(informative)Precisiondata... 17 Annex D (informative) Testing salt water samples with the luminescent bacteria test with liquid-dried bacteria.... .18 Bibliography 21 CopyrightInternatinal Oorganizain for standardizalion ghts reserved ii nse from IHS Not for Resale ISO 11348-2:2007(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through IsO technical committees. Each member body interested in a subject for which a technical committee has been non-governmental, in liaison with isO, also take part in the work. isO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2 The main task of technical committees is to prepare International Standards. Draft International Standards adopted bythe technical committees are circulated to the member bodies for voting.Publication as ar International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ISO 11348-2 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. This second edition cancels and replaces the first edition (IsO 11348-2:1998), which has been technically revised. Iso 11348 consists of the following parts, under the general title Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischer