ISO INTERNATIONAL STANDARD 11348-1 Second edition 2007-12-01 Water quality Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) - Part 1: Method using freshly prepared bacteria Qualite de I'eau Determination de I'effet inhibiteur d'eéchantillons d'eau sur la luminescence de Vibrio fischeri (Essai de bactéries luminescentes)- Partie 1: Methode utilisant des bacteries fraichement preparees Reference number ISO 11348-1:2007(E) @ ISO 2007 HS under I without license from IHS Not for Resale ISO 11348-1:2007(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTEDDOCUMENT @ISO2007 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2007 - All rights reserved tedwithoutlicensefrom IHS Not for Resale ISO 11348-1:2007(E) Contents Page Foreword iv Introduction 1 Scope 2 Normative references 3 Principle. 4 Interferences 5 Reagents and materials 6 Apparatus.... 7 Sampling and sample pretreatment... 8 Cultivation of luminescent bacteria 9 Procedure 10 Evaluation. 11 Expression of results 10 12 Criteria of validity. 11 13 Precision. 12 14 Test report. AnnexA(informative)Colour-correctionmethod... Annex B (informative) Dilution level D - Preparation of the dilution series..... Annex C (informative) Precision data. cultured bacteria... 20 Bibliography. Copyright International Organization for Standardizalon ghts reserved ii se from IHS Not for Resale ISO 11348-1:2007(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies technicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeer non-governmental, in liaison with isO, also take part in the work. iso collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/lECDirectives,Part2 adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 11348-1 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. This second edition cancels and replaces the first edition (IsO 11348-1:1998), which has been technically revised. ISsO 11348 consists of the following parts, under the general title Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test): Part 1: Method using freshly prepared bacteria Part 2: Method using liquid-dried bacteria Part3:Methodusing freeze-driedbacteria lal Organization for Standardizatio ISO2007-All righ