ISO INTERNATIONAL STANDARD 10706 First edition 2000-04-01 Water quality Determination of long term toxicity of substances to Daphnia magna Straus (Cladocera, Crustacea) Qualité de I'eau Deétermination de la toxicite a long terme de substances vis-a-vis de Daphnia magna Straus (Cladocera, Crustacea) Reference number ISO 10706:2000(E) ISO @ ISO 2000 py IHS under ted without license from IHS Not for Resale ISO 10706:2000(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by isO member bodies. In the unlikely event ?ISO2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 734 10 79 E-mail [email protected] Web www.iso.ch Printed in Switzerland @ Iso 2000 - All rights reserved ermittedwithoutlicense from IHS Not for Resale ISO 10706:2000(E) Contents Page Foreword iv Introduction 1 Scope 2 Normative references. 3 Principle 4 Test environment.. 5 Reagents and materials 6 Apparatus... < Treatment and preparation of samples ... 7.1 Special precautions for sampling and transportation of water or effluent samples, 7.2 Preparationofsolutionsofsubstancestobetested..... 8 Procedure.. 8.1 Controls .... 8.2 Selection of exposure concentrations 8.3 Renewal of exposure solutions.. 8.4 Introduction of animals into the test system. 8.5 Feeding of organisms ... 8.6 Observations and measurements, 8.7 Validity of the test.. 9 Data analysis and expression of results ... .6 10 Test report. Annex A (informative) OECD M4 and M7 media — Preparation and acclimation of Elendt M4 and M7 media......... 9 AnnexB(informative)AsTMreconstitutedhardfreshwater.. 12 Annex C (informative) Data collection sheet.. 13 Annex D (informative) Calculation of time-weighted mean concentration. 15 Bibliography. ili THS unde nse from IHS Not for Resale ISO 10706:2000(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO committees. Each member body interested in a subject for which a technical committee has been established has liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights International Standard ISO 10706 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Annexes A, B, C and D of this International Standard are for information only ional Organization for Standardizatior ISO2000-Allrightsreserved ISO 10706:2000(E) Introduction This International Standard defines a procedure for the determination of the long term sublethal toxicity of guideline produced by the Organisation for Economic Co-operation and Development (OECD Guideline 211, see reference [1] in the Bibliography]. V Not for Resale aadizao No reproductic