ISO INTERNATIONAL STANDARD 10705-3 First edition 2003-10-01 Water quality Detection and enumeration of bacteriophages Part 3: Validation of methods for concentration of bacteriophages from water Qualité de I'eau Détection et dénombrement des bacteriophages - Partie 3: Validation des methodes de concentration des bacteriophages dansIeau Reference number ISO 10705-3:2003(E) @ ISO 2003 ISO 10705-3:2003(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The IsO Central Secretariat accepts no liability in this area. Adobe isatrademarkof AdobeSystems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation @ISO2003 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's memberbody in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ ISO 2003 - All rights reserved ISO 10705-3:2003(E) Contents Page Foreword iv 1 Scope. 2 Normative references . 3 Terms and definitions.. 4 Principle .. 5 Reagents. 9 Apparatus and glassware. 7 Sampling. 8 Preparation of sewage samples for spiking.. 9 Procedure 4 10 Calculation 11 Analytical quality control .. 12 Test report.. Annex A (informative) Recommended methods for concentration of bacteriophages from water depending on the volume, turbidity and particle content... 8 Annex B (informative) Example of a validation process of a concentration method.. Bibliography @ ISO 2003 - All rights reserved ili ISO 10705-3:2003(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies non-governmental, in liaison with isO, also take part in the work. isoO collaborates closely with the InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote ISO 10705-3 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4, Microbiological methods. Iso 10705 consists of the following parts, under the general title Water quality - Detection and enumeration of bacteriophages: Part 1: Enumeration of F-specific RNA bacteriophages Part2:Enumerationof somaticcoliphages Part 3:Validation of methods for concentration of bacteriophages from water Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis iv @ ISO 2003 - All rights reserved INTERNATIONAL STANDARD ISO 10705-3:2003(E) Water quality Detection and enumeration of bacteriophages - Part 3: Validation of methods for concentration of bacteriophages from water WARNING- Persons using this part of Iso 107o5 should be familiar with normal laboratory practice This part of Iso 10705 does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is imperative that personnel involved in validation of methods for concentration of bacteriophages from water have relevant experience with the methods of enumeration of bacteriophages (see ISO/TR 13843[1]). Scope This part of isO 10705 specifies the general princi