ISO INTERNATIONAL STANDARD 10705-2 First edition 2000-04-01 Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of somatic coliphages Qualite de I'eau Détection et denombrement des bacteriophages - Partie 2: Dénombrement des coliphages somatiques Reference number E ISO 10705-2:2000(E) ISO @ ISO 2000 py IHS under ted without license from IHS Not for Resale ISO 10705-2:2000(E) PDFdisclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISo Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by isO member bodies. In the unlikely event @ISO2000 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 734 10 79 E-mail [email protected] Web www.iso.ch Printed in Switzerland @ Iso 2000 - All rights reserved py IHS unde ermittedwithoutlicense from IHS Not for Resale ISO 10705-2:2000(E) Contents Page 1 Scope 2 Normative references .. 3 Terms and definitions 4 Safety precautions.. 5 Principle. 6 Diluent, culture media and reagents 6.1 Basic materials.... 6.2 Diluent. 7 Apparatus and glassware, 8 Microbiological reference cultures. Sampling. 10 Preparation of test material.. 10.1 Culturing and maintenance of host strains 10.2 Calibration of absorbance measurements for counts of viablemicroorganism 11 Procedure. 11.1 Preparation of inoculum cultures 11.2 Standard procedure.. 11.3 Method for samples with high bacterial background flora, 11.4 Samples with low phage counts ... 11.5 Presence/absence test... 11.6 Quality assurance. 12 Expression of results . 12.1 Plaque count procedures (11.2 to 11.4).. 12.2 Presence/absence test (11.5).. 13 Test report. 10 Annex A (normative) Culture media, reagents and diluent. Annex B (informative) General description of somatic bacteriophages 14 Annex C (informative) Culturing of bacteriophage X174 15 Bibliography... 16 py IHS unde nse from IHS Not for Resale ISO 10705-2:2000(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO committees. Each member body interested in a subject for which a technical committee has been established has liaison with IsO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote Attention is drawn to the possibility that some of the elements of this part of IsO 10705 may be the subject of International Standard ISO 10705-2 was prepared by Technical Committee ISO/TC 147, Water quality Subcommittee SC 4, Microbiological methods. isO 10705 consists of the following parts, under the general title Water quality- Detection and enumeration of bacteriophages: —Part 1: Enumeration of F-specific RNA bacteriophages !Part2:Enumeration of somatic coliphages Part 3: Concentration methods Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis ional Organizationfor Standardization @ ISO 2000 - All