ISO INTERNATIONAL STANDARD 10540-1 First edition 2003-02-01 Animal and vegetable fats and oils - Determination of phosphorus content Part 1: Colorimetric method Corps gras d'origines animale et vegétale Détermination de la teneur enphosphore Partie 1: Méthode colorimétrique Reference number ISO 10540-1:2003(E) ISO @ISO2003 by IHS under lic itted without license from IHS Not for Resale ISO 10540-1:2003(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ?ISO2003 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2003 - All rights reserved ermittedwithoutlicense from IHS Not for Resale ISO 10540-1:2003(E) Foreword ISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnational standardsbodies(iSo committees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhas liaison with sO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEc Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights IsO shall not be held responsible for identifying any or all such patent rights. vegetable fats and oils. Determination of phosphorus content: — Part 1: Colorimetric method Part 2: Method using graphite furnace atomic absorption spectrometry Part 3:Method using inductively coupled plasma (IcP) optical emission spectroscopy ii aadizao No reproductic neworkingmitedwithout licenefrmH Not for Resale INTERNATIONAL STANDARD ISO 10540-1:2003(E) Animal and vegetable fats and oils Determination of phosphorus content - Part 1: Colorimetric method Scope This part of isO 10540 specifies a colorimetric method for the determination of the phosphorus content of animal and vegetable oilsandfats This method is not suitable for determining the phosphorus content of commercial lecithin as this requires an ashing temperature of 800 °C. 2 Principle The test portion is charred (carbonized) in the presence of magnesium hydroxycarbonate and then ashed. the molybdenum blue method. 3 Reagents Use only reagents of recognized analytical grade, unless otherwise stated. 3.1 Magnesium hydroxycarbonate, [(MgCO3)n-Mg(OH)2]-H2O, with a magnesium oxide content of between 40 % and 46 % (by mass). Magnesium carbonate, hydrated, basic, [(MgCO3)4-Mg(OH)2]-5H2O, is suitable. 3.2Hydrochloric acid, c(HCl) = 2 mol/l. 3.3Sodium hydroxide solution, c(NaOH) = 5mol/l. 3.4 Reducing solution. Weigh out 0,500 g of p-methylaminophenol sulfate [(HOCH4NHCH3)2:H2SO4l, 2,5 g of so